Sugar co-transport with H+ in Vinca protoplasts: Inhibition of the uptake by ATPase inhibitors

  • Suzuki M
  • 1

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

Sugar uptake by protoplasts isolated from suspension cultured cells of Vinca rosea was studied. Several respiratory inhibitors including sodium azide, iodoacetate, and dinitrophenol inhibited D-glucose uptake. The uptake also declined at low temperature. This evidence suggests that the uptake by protoplasts depends on cellular energy. D-Glucose, D-fructose and sucrose showed alkalinization of the medium, suggesting that protons entered into cells together with these sugars. In contrast, sorbitol induced very little alkalinization and inositol as well as dextran showed no alkalinization of the medium. Diethylstilbestrol (DES) and vanadate, an inhibitor of plasmalemma ATPase, depressed the uptake of D-glucose by protoplasts. This suggests the involvement of plasmalemma ATPase in D-glucose-cotransport with protons. Similar inhibition of D-glucose uptake was observed with N,N???-dicyclohexyl carbodiimide (DCCD), an inhibitor of energy transfer, and oligomycin, an inhibitor of mitochondrial ATPase. The presence of KCl weakened the effect of DES, DCCD and vanadate, but not of oligomycin. ?? 1982.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Masahiko Suzuki

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free