Suitability of chromogenic substrates for Alcalase detection for inclusion in an aerosol monitoring system

  • Nitescu I
  • Cumming R
  • Rowell F
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 7

    Citations

    Citations of this article.

Abstract

The suitability of several chromogenic substrates to detect Alcalase (Novo) and other protease enzymes in sensing devices was evaluated. The sensitivity of the substrates was evaluated from their K(cat) values and their stability to spontaneous breakdown was determined over an 8-h period. p-Nitrophenyl trimethylacetate showed reasonable sensitivity but was the least stable of the substrates. The other substrates were based on p-nitroanalide chromophores and were very stable. The substrate which showed the highest sensitivity to Alcalase was N-succinyl-L-alanyl-L-alanyl-L-ananyl-p-nitroanilide (k3= 50.25 μmol AU-1min-1). This substrate was selected for an 8-h assay. Esperase (Novo) and Subtilisin Carlsberg were compared with Alcalase with the substrate p-nitrophenyl trimethylacetate. A further evaluation of N-succinyl-L-alanyl-L-alanyl-L-alanyl-p-nitroanilide was made by incubating this substrate with Alcalase over an 8-h period. There was a gradual reduction in the rate over the 8-h period but it is suggested that the enzyme-substrate reaction is sufficiently stable for incorporation in a sensor for long-term monitoring.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • I. Nitescu

  • R. H. Cumming

  • F. J. Rowell

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free