Synchronization Of Cell Cultures Of Higher Plants

Abstract

This chapter discusses the main methods used for synchronization of cells from higher plants. The chapter provides an outline of procedures for synchronization by starvation and refeeding of phosphate or auxin in Catharanthus roseus cells. Synchronization by the phosphate starvation method had been considered to be restricted to suspension cultures of C. roseus cells. Recent work indicates that synchrony can be achieved by the phosphate starvation method in suspension cultures of Medicago varia and of Populus alba. The formulation of the basal culture medium may be a key factor for achieving synchrony by the phosphate starvation method. All suspension cultures amenable to synchronization by this method are maintained in Murashige–Skoog (MS) medium. Synchronization by auxin starvation has been reported in suspension cultures of carrot cells and of C. roseus cells but strains suitable for synchronization by this means, are limited. Synchronization by aphidicolin treatment has been reported in suspension cultures of carrot, tobacco, and rice. © 1995, Academic Press Inc.