Synthesis and enzymatic incorporation of morpholino thymidine-5'- triphosphate in DNA fragments

12Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydroxymethyl)morpholine-6- triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1- (β-D-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide analogue into DNA fragments was investigated Morpholino thymidine- 5'-triphosphate was incorporated in a base-specific process and acted as a novel chain terminator in DNA sequencing, similarly to the corresponding dideoxynucleotide.

Cite

CITATION STYLE

APA

Marciacq, F., Sauvaigo, S., Issartel, J. P., Mouret, J. F., & Molko, D. (1999). Synthesis and enzymatic incorporation of morpholino thymidine-5’- triphosphate in DNA fragments. Tetrahedron Letters, 40(25), 4673–4676. https://doi.org/10.1016/S0040-4039(99)00842-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free