Tachykinin NK2 receptors facilitate acetylcholine release from guinea-pig isolated trachea

  • D'Agostino G
  • Erbelding D
  • Kilbinger H
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Abstract

The release of newly synthesised [3H]acetylcholine was evoked by electrical field stimulation (5 Hz, 600 pulses) of epithelium-deprived guinea-pig trachea strips after sensory neuropeptides depletion with 3 μM capsaicin. The selective tachykinin NK2receptor agonist [βAla8]neurokinin A-(4-10) increased in a concentration-dependent manner the electrically-induced release of [3H]acetylcholine. The facilitatory effect was antagonised by the selective non-peptide tachykinin NK2receptor antagonist, SR 48968 (apparent pK(B) 8.9). The tachykinin NK1and NK3receptor agonists substance P methyl ester and senktide (both 10 and 100 nM), respectively, did not affect the evoked release of [3H]acetylcholine. It is concluded that the cholinergic nerves of guinea-pig trachea are endowed with prejunctional facilitatory tachykinin receptors of the NK2subtype. Copyright (C) 2000 Elsevier Science B.V.

Author-supplied keywords

  • Acetylcholine release
  • SR 48968
  • Tachykinin NK2receptor
  • Trachea
  • [βAla8]Neurokinin A-(4-10)

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Authors

  • Gianluigi D'Agostino

  • Doris Erbelding

  • Heinz Kilbinger

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