The thermodynamic characteristic of four-heme cytochrome c in Rhodopseudomonas viridis reaction centers, as derived from a quantitative analysis of the differential absorption spectra in α-domain

  • Shinkarev V
  • Drachev A
  • Dracheva S
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Abstract

A method of decomposing of the absorption spectrum of four-heme cytochrome of a Rhodopseudomonas viridis reaction center preparation into spectra of individual components was used to estimate the degree of the reduction of hemes as a function of redox potential in the medium. The method enables an evaluation of the shape of redox-titration curves of each heme. The redox-titration curves derived by this approach are approximated well by a Nernst equation with n = 1 and Em-values of 360 mV, 312 mV, 20 mV and less than -50 mV. For all of the redox species the values of midpoint potential estimates by the above method are in good agreement with those determined earlier using another procedure [Dracheva et al. (1988) Eur. J. Biochem. 171, 253-264]. The accuracy of deconvolution of data is within the experimental errors of the redox potential measurement. © 1990.

Author-supplied keywords

  • (Rhodopseudomonas viridis)
  • Deconvolution method
  • Four-heme cytochrome
  • Reaction center
  • Redox titration

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Authors

  • V. P. Shinkarev

  • A. L. Drachev

  • S. M. Dracheva

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