A method of decomposing of the absorption spectrum of four-heme cytochrome of a Rhodopseudomonas viridis reaction center preparation into spectra of individual components was used to estimate the degree of the reduction of hemes as a function of redox potential in the medium. The method enables an evaluation of the shape of redox-titration curves of each heme. The redox-titration curves derived by this approach are approximated well by a Nernst equation with n = 1 and Em-values of 360 mV, 312 mV, 20 mV and less than -50 mV. For all of the redox species the values of midpoint potential estimates by the above method are in good agreement with those determined earlier using another procedure [Dracheva et al. (1988) Eur. J. Biochem. 171, 253-264]. The accuracy of deconvolution of data is within the experimental errors of the redox potential measurement. © 1990.




Shinkarev, V. P., Drachev, A. L., & Dracheva, S. M. (1990). The thermodynamic characteristic of four-heme cytochrome c in Rhodopseudomonas viridis reaction centers, as derived from a quantitative analysis of the differential absorption spectra in α-domain. FEBS Letters, 261(1), 11–13. https://doi.org/10.1016/0014-5793(90)80624-R

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free