Thiocyanate stabilizes AMPA binding to the quisqualate receptor

  • Nielsen E
  • Cha J
  • Honoré T
 et al. 
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Abstract

Calcium and chloride ions stimulated [3H]glutamate binding to quisqualate-sensitive [3H]glutamate binding sites 4-fold, as measured by quantitative autoradiography, whereas 100 mM potassium thiocyanate had no additional effect. In contrast, calcium and chloride had little effect on the binding of [3H](RS)-α-amino-3-hydroxy-5-methylisoxazole-4-proprion ic acid ([3H]AMPA), but 100 mM thiocyanate stimulated binding 4-fold. AMPA displace little [3H]glutamate binding from quisqualate-sensitive binding sites in the molecular layer of the cerebellum in the absence of thiocyanate. However, in the presence of thiocyanate AMPA became a more effective displacer, but still displaced only 44% of the quisqualate-sensitive [3H]glutamate binding. The distribution of [3H]glutamate binding to quisqualate-sensitive sites was similar to but not identical with that of [3H]AMPA binding. However, the distribution of AMPA-displaceable [3H]glutamate binding correlated highly (r = 0.97, P

Author-supplied keywords

  • (Autoradiography, Receptor, Rat)
  • Thiocyanate
  • [3H]AMPA binding
  • [3H]Glutamate binding

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Authors

  • Elsebet Nielsen

  • Jang Ho J. Cha

  • Tage Honoré

  • John B. Penney

  • Anne B. Young

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