Transport of the antibacterial agent (6S)-6-fluoroshikimate and other shikimate analogues by the shikimate transport system of Escherichia coli

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Abstract

We show that the antibacterial agent, (6S)-6-fluoroshikimate, is a substrate for the shikimate transport system of Escherichia coli because in exchange-diffusion experiments it displaced intracellular [14C]shikimate with the same kinetics as did unlabelled shikimate. Other shikimate analogues were also substrates: as judged by similar experiments or, in the case of (6R)-6-fluoroshikimate, by inference.

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Jude, D. A., Ewart, C. D. C., Thain, J. L., Davies, G. M., & Nichols, W. W. (1996). Transport of the antibacterial agent (6S)-6-fluoroshikimate and other shikimate analogues by the shikimate transport system of Escherichia coli. Biochimica et Biophysica Acta - Biomembranes, 1279(2), 125–129. https://doi.org/10.1016/0005-2736(95)00295-2

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