Trypanosoma rhodesiense was maintained for three serial passages in embryonated chicken eggs. Rapid passage in mice was used to prepare trypanosome suspensions, from each passage, and from the original population, for agglutination tests. Antisera to the various trypanosome populations studied were prepared in rats. The four antigens and all antisera were crosstested by the direct agglutination test. With a single exception all reactions were positive. It is concluded that no antigenic change in the trypanosomes had occurred during passage in eggs. © 1971.
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