Two methods for the separation of α-fetoprotein (AFB) and albumin using (A) affinity chromatography on Blue Sepharose CL-6B and (B) ampholyte displacement chromatography are described. The heterogeneity of immunoreactive human AFP is demonstrated by ampholyte displacement chromatography; possibly seven variants of AFP can be distinguished by this method. © 1978.
Young, J. L., & Webb, B. A. (1978). Two methods for the separation of human α-fetoprotein and albumin. (A) Affinity chromatography using blue sepharose CL-6B and (B) ampholyte displacement chromatography. Analytical Biochemistry, 88(2), 619–623. https://doi.org/10.1016/0003-2697(78)90464-5