Two-Dimensional Gel Electrophoretic Analysis of Chromatin-associated Proteins from Leaves of Different Species and Varieties of Nicotiana

  • van Telgen H
  • van Loon L
  • 1


    Mendeley users who have this article in their library.
  • 10


    Citations of this article.


From different Nicotiana species (N. sylvestris Spegaz. et Comes, N. glutinosa L., and N. tabacum L. cultivars) which differ in their sensitivity towards tobacco mosaic virus (TMV), chromatin-associated protein profiles were compared by two-dimensional polyacrylamide gel electrophoresis. After removal of DNA from dissociated chromatin preparations, the remaining chromatin-associated proteins were extracted with phenol and precipitated with ethanol. The sensitive silver staining of the gel enabled visualization of up to 350 different polypeptides. Comparative analysis showed that over 90% of the polypeptides were similar in all Nicotiana species but discrete differences provided unique patterns for each of the species and cultivars examined. However, no specific polypeptide spot could be associated with the presence of the gene N, which governs hypersensitivity towards TMV. Nevertheless, the method presented here allows study of the chromatin proteins involved in the regulation of gene expression during growth and development, as well as analyses of the influence of environmental factors on genetic regulatory elements. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved.

Author-supplied keywords

  • 2-DE
  • BPB
  • IEF
  • N,N,N',N'-tetramethylethylenediamine
  • NP-40
  • Nicotiana
  • Nonidet P-40
  • SDS
  • TMV
  • Two-dimensional gel electrophoresis
  • bromo phenol blue
  • isoelectric focusing
  • leaf chromatin proteins
  • sodium dodecyl sulphate
  • tobacco mosaic virus
  • two-dimensional gel electrophoresis

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • H. J. van Telgen

  • L. C. van Loon

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free