Uptake and metabolism of [3h]ecdysone in cultured ovaries of the silkworm, Bombyx mori

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Abstract

Uptake of ecdysone by ovaries of the silkworm, Bombyx mori, was studied by organ culture. [3H]Ecdysone was transported almost linearly into the ovary for up to 3 h of the incubation. The uptake was proportional to the concentration of the labeled ecdysone, unsaturably, at concentrations ranging up to 10-6M. Ecdysone which had been transported into the ovary could usually be removed when the ovary was re-transferred to the ecdysone-free medium. Analysis of the transported compounds by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the marked conversion of ecdysone into 20-hydroxyecdysone, unknown metabolites and conjugated forms. Physiological significance of the metabolic activity of the ovary is discussed with respect to the accumulation of ecdysteroids in the ovary. © 1984.

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Manabu, O., & Eiji, O. (1984). Uptake and metabolism of [3h]ecdysone in cultured ovaries of the silkworm, Bombyx mori. Molecular and Cellular Endocrinology, 38(1), 13–19. https://doi.org/10.1016/0303-7207(84)90140-0

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