Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research

  • Brindle E
  • Miller R
  • Shofer J
 et al. 
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Abstract

Objective: We developed assays for measurement of urinary βLH and βFSH under collection and storage conditions typical of non-clinical research settings. Design and methods: IEMAs for free βLH and total βFSH were validated by standard methods. Stability of urinary βLH and βFSH was tested across freeze-thaws and stored long term at 4°C or - 20°C, or short term at room temperature, and with heating to dissociate the subunits. Results: The IEMAs exhibited acceptable parallelism, specificity, recovery (averaging 100% for βLH, 97% for βFSH), imprecision (maximum within-run and between run CVs, respectively, 4.8% and 25.7% for βLH, 5.6% and 17.0% for βFSH), and minimum detectable dose (2.5 pmol/L for βLH, 6.8 pmol/L for βFSH). Urine and serum measures were highly correlated (r = 0.95 for LH, 0.86 for FSH). There was no consistent decline with any storage type. Dissociation of subunits by heating was needed for βLH, but not βFSH. Conclusion: These IEMAs measure free βLH and total βFSH, overcoming inter-individual variability in, and collection and storage effects on, subunit dissociation, without the need for urine preservatives. © 2006 The Canadian Society of Clinical Chemists.

Author-supplied keywords

  • IEMA
  • Stability
  • βFSH
  • βLH

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