Vaccination of pigs against pseudorabies virus with plasmid DNA encoding glycoprotein D

  • Haagmans B
  • Van Rooij E
  • Dubelaar M
 et al. 
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Abstract

We analysed the ability of a plasmid carrying the gene encoding glycoprotein D (gD) of pseudorabies virus (PRV) to induce humoral and cell- mediated immune responses and assessed the protection provided by PRV-gD DNA vaccination against challenge infection with PRV. Immunization with plasmid PRV-gD induced neutralizing antibodies and lymphocyte proliferative responses both in mice and pigs. Moreover, when challenged with virulent PRV six weeks following the last immunization, PRV-gD DNA vaccinated pigs excreted virus for a significantly shorter period and showed less clinical symptoms than pigs vaccinated with a control plasmid. Thus, in the target animal, DNA vaccination with PRV-gD DNA induces protective immunity against challenge infection.

Author-supplied keywords

  • DNA vaccination
  • Glycoprotein D
  • Pseudorabies virus

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Authors

  • Bart L. Haagmans

  • Eugene M A Van Rooij

  • Mark Dubelaar

  • Tjeerd G. Kimman

  • Marian C. Horzinek

  • Virgil E C J Schijns

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