Variation in the pattern of [Ca2+]i change induced by acetylcholine in cultured hippocampal neurons

  • Iijima T
  • Kudo Y
  • Ogura A
 et al. 
  • 1

    Readers

    Mendeley users who have this article in their library.
  • 9

    Citations

    Citations of this article.

Abstract

Acetylcholine (ACh) caused various patterns of change in the intracellular Ca2+concentration ([Ca2+]i) in cultured rat hippocampal neurons. We studied the underlying mechanisms of the [Ca2+]ichanges with simultaneous recording of [Ca2+iand membrane potential/current. In most cases, [Ca2+]irise was accompanied by a membrane depolarization. The [Ca2+]ichange was significantly reduced when the membrane was voltage clamped, which implies that most of the [Ca2+]irise results from the Ca2+influx through the voltate-gated Ca2+channel activated by the membrane depolarization. The membrane depolarizations were classified into two types, one associated with membrane conductance decrease and the other associated with membrane conductance increase. The former results from potassium conductance ((gK+) decrease, and the latter may result from the activation of a Na+-permeable channel. However, [Ca2+]ielevation was also observed in some neurons showing membrane hyperpolarization in response to ACh. This seems to show that ACh liberates Ca2+from the intracellular Ca2+store, resulting in the activation of a calcium-dependent K+channel (KCa). The variations of ACh in the hippocampal neurons seem to result from a variety of muscarinic acetylcholine receptors and various species of ion channels governed by those receptors. © 1990.

Author-supplied keywords

  • Acetylcholine
  • Calcium-dependent K+-channel
  • Hippocampal neuron
  • Inositol triphosphate
  • Intracellular calcium
  • M-channel

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Toshio Iijima

  • Yoshihisa Kudo

  • Akihiko Ogura

  • Kyoto Akita

  • Gen Matsumoto

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free