A vasoactive peptide, endothelin-3, is produced by and specifically binds to primary astrocytes

  • Ehrenreich H
  • Kerhl J
  • Anderson R
 et al. 
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Abstract

Primary rat astrocytes were found by immunohistochemistry to display positive staining for endothelin-3, located predominantly in the perinuclear area. The ability of these cells to produce and release endothelin-3 was confirmed by a combination of reverse-phase HPLC and radioimmunoassay, specific for endothelin-3, which demonstrated immunoreactive peptide in cellular extracts and astrocyte-conditioned medium. In addition, astrocytes were shown to possess a single class of binding sites for endothelin with comparable high affinity for endothelin-1, -2 and -3. These results suggests that astrocytes, by virtue of their ability to produce and secrete endothelin-3, serve as a potential extravascular source of intracerebral vasoregulation capable of influencing regional cerebral blood flow. © 1991.

Author-supplied keywords

  • Astrocyte
  • Brain
  • Endothelin-3
  • Endothelin-receptor
  • Glial cell
  • Vasoactive peptide

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Authors

  • Hannelore Ehrenreich

  • John H. Kerhl

  • Robert W. Anderson

  • Peter Rieckmann

  • Ljubisa Vitkovic

  • John E. Coligan

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