A vasoactive peptide, endothelin-3, is produced by and specifically binds to primary astrocytes

  • Ehrenreich H
  • Kerhl J
  • Anderson R
 et al. 
  • 4


    Mendeley users who have this article in their library.
  • 107


    Citations of this article.


Primary rat astrocytes were found by immunohistochemistry to display positive staining for endothelin-3, located predominantly in the perinuclear area. The ability of these cells to produce and release endothelin-3 was confirmed by a combination of reverse-phase HPLC and radioimmunoassay, specific for endothelin-3, which demonstrated immunoreactive peptide in cellular extracts and astrocyte-conditioned medium. In addition, astrocytes were shown to possess a single class of binding sites for endothelin with comparable high affinity for endothelin-1, -2 and -3. These results suggests that astrocytes, by virtue of their ability to produce and secrete endothelin-3, serve as a potential extravascular source of intracerebral vasoregulation capable of influencing regional cerebral blood flow. © 1991.

Author-supplied keywords

  • Astrocyte
  • Brain
  • Endothelin-3
  • Endothelin-receptor
  • Glial cell
  • Vasoactive peptide

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Hannelore Ehrenreich

  • John H. Kerhl

  • Robert W. Anderson

  • Peter Rieckmann

  • Ljubisa Vitkovic

  • John E. Coligan

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free