In vitro assembly of the outer capsid of bovine rotavirus is calcium-dependent

  • Ready K
  • Sabara M
  • Babiuk L
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Abstract

The nucleocapsid protein (VP6) and outer capsid glycoprotein (VP7) of bovine rotavirus (BRV) assemble in vitro, in 0.01 M Tris-HCl, pH 8.0, 50 mM CaCl2, into smooth particles resembling double-shelled BRV. That the two proteins interact is demonstrated by the immunoprecipitation of both by antibody directed against either VP6 or VP7. The calcium-dependence, particle morphology, and immunoreactivity in ELISA suggest that VP7 is presented authentically on the outer capsid. The implications for rotavirus morphogenesis are discussed. © 1988.

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