The nucleocapsid protein (VP6) and outer capsid glycoprotein (VP7) of bovine rotavirus (BRV) assemble in vitro, in 0.01 M Tris-HCl, pH 8.0, 50 mM CaCl2, into smooth particles resembling double-shelled BRV. That the two proteins interact is demonstrated by the immunoprecipitation of both by antibody directed against either VP6 or VP7. The calcium-dependence, particle morphology, and immunoreactivity in ELISA suggest that VP7 is presented authentically on the outer capsid. The implications for rotavirus morphogenesis are discussed. © 1988.
Ready, K. F. M., Sabara, M. I. J., & Babiuk, L. A. (1988). In vitro assembly of the outer capsid of bovine rotavirus is calcium-dependent. Virology, 167(1), 269–273. https://doi.org/10.1016/0042-6822(88)90077-3