In vitro synthesis of chloroplast ferredoxin as a high molecular weight precursor in a cell-free protein synthesizing system from wheat germs

  • Huisman J
  • Moorman A
  • Verkley F
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Abstract

RNA isolated from leaves of Nicotiana tabacum, etiolated leaves of Phaseolus vulgaris and from cells of Chlamydomonas reinhardii, can be translated in cell-free extracts derived from wheat germs. The in vitro products were identified by SDS electrophoresis after immunoprecipitation with specific antisera against ferredoxin. The in vitro products appeared to have a Mr20,500, in contrast to authentic ferredoxin that has a Mr17,000. We propose that chloroplast ferredoxin is synthesized in vitro as a biosynthetic precursor. Ferredoxin synthesis was found to be associated with poly(A)-RNA, which supports our previous conclusion that ferredoxin messenger is a transcript from the DNA of the nucleus. © 1978.

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Authors

  • J. G. Huisman

  • A. F.M. Moorman

  • F. N. Verkley

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