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Ewoud B. compeer

  • PhD
  • Postdoc
  • University of Oxford
  • 11h-indexImpact measure calculated using publication and citation counts. Updated daily.
  • 413CitationsNumber of citations received by Ewoud B.'s publications. Updated daily.

About

My primary research goals are to understand the role of endocytic network remodeling and reorganization in immune cell-cell interaction using leading microscopy techniques and its translation to human diseases. As an undergraduate, I was able to conduct research with Dr. Berend-Jan Bosch on the mechanisms of Coronavirus entry during endocytosis, and with Prof. Cox Terhorst in Boston, on the microbial-sensing role of SLAM and the consequences for bacterial endosome function in macrophages. As a PhD candidate with Dr. Marianne Boes, my research focused on cell biological processes that occur during/prior to activation of antigen-specific lymphocytes with a special focus to endosomal remodeling and reorganization. I gained expertise in the isolation, culturing, and live cell imaging of human immune cells and their endocytic network. I developed a novel protocol to monitor the remodeling of recycling endosomes in live human dendritic cells. I also showed that a genetic mutation found in BLK of a CVID patients accelerated endosomal destruction of B cell receptor-internalized antigen and reduced ability to elicit CD40L-expression on antigen-specific CD4 T cells. In my first postdoc, I expanded my live cell imaging skills with various optogenetic tools. Specifically imaging beyond the light diffraction limit, including SPT-PALM, 2D-PALM, and dSTORM at the EMBL node and ARC center of excellence for Single Molecule imaging at UNSW, Sydney. Applying these techniques on T cell receptor (TCR) trafficking, we identified a protein that is pivotal to activation-induced endocytic network reorganization required for efficient T cell activation. Interestingly, it seems that endocytic trafficking coordinates TCR's nanoscalled surface distribution. In my second postdoc, I combine my expertise in live cell and super-resolution imaging with my extensive knowledge of the endocytic network and associated signaling pathways to investigate the recently identified TCR-enriched extracellular vesicles released in the immunological synapse of helper T cells.

Recent publications

  • Maintenance of CD4 T cell fitness through regulation of Foxo1

    • Newton R
    • Shrestha S
    • Sullivan J
    • et al.
    N/AReaders
    10Citations
  • Splenic TFH expansion participates in B-cell differentiation and antiplatelet-antibody production during immune thrombocytopenia

    • Audia S
    • Rossato M
    • Santegoets K
    • et al.
    N/AReaders
    28Citations

Professional experience

Postdoc

University of Oxford

May 2017 - Present

Education

Laboratory of Translational Immunology

University Medical Center Utrecht

January 2010 - May 2015(5 years)