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Marisa Lopes de Sousa

  • PhD
  • Post-Doc Researcher
  • NOVA Medical School/CEDOC-Nova University of Lisbon
  • 0ReadersNumber of instances of Marisa's publications in Mendeley libraries. Updated daily.
  • 11PublicationsNumber of items in Marisa's My Publications folder on Mendeley.

Research interests

The main goal of my research is to determine the efficacy of marine drugs on modulation of ion channels involved in Chronic pain.

About

The main scientific skills and competences I've developed during my research are the following: Molecular biology: Cloning, Mutagenesis, PCR, Lipofection and Electroporation. Cell Culture: Production of stably transfected Cell Lines; Production of Primary Cell Cultures – from human, pig and murine airways; Establishment of Organotypic Cultures. Electrophysiology: Micro-Ussing chamber in Native Epithelia (human/ murine airways and colon) and Cell Monolayers (Cell lines and primary organotypic cultures); Iodide Efflux (Cell lines); and Halide-eYFP sensitive Iodide Influx (Kinetics in Microplate reader); Patch-clamp (Port-a-patch). Biochemical and Cell Biology: Western blot; Co-IP; Metabolic Pulse-Chase (with Radioactive labeling); Immunofluorescence (Cells and Tissues); Histopathology (Frozen and paraffin embed tissue preparation, cutting and histology staining); Confocal Microscopy; ELISA; Flow Citometry. Statistical tools: Descriptive statistics; Parametric tests for Independent (or paired) samples (t-Student test; ANOVA; ANCOVA, post-hoc tests); Classification analysis (Clusters and Discriminant analysis); Linear and Non-linear Regression; Mixed Models - Linear Regression.

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Publications (5)

  • Comparative ex vivo, in vitro and in silico analyses of a CFTR splicing mutation: Importance of functional studies to establish disease liability of mutations.

    • Ramalho A
    • Clarke L
    • Sousa M
    • et al.
    N/AReaders
    N/ACitations
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  • Appearance of de novo dysplastic spitzoid compound naevus in an adalimumab-treated psoriatic patient: case report and review of the possible causal relationship with TNF-α blockers.

    • Sousa M
    • Freitas J
    • Antunes J
    • et al.
    N/AReaders
    N/ACitations
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  • High-content siRNA screen reveals global ENaC regulators and potential cystic fibrosis therapy targets.

    • Almaça J
    • Faria D
    • Sousa M
    • et al.
    N/AReaders
    N/ACitations
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  • Rectal forceps biopsy procedure in cystic fibrosis: technical aspects and patients perspective for clinical trials feasibility.

    • Servidoni M
    • Sousa M
    • Vinagre A
    • et al.
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  • Revertants, low temperature, and correctors reveal the mechanism of F508del-CFTR rescue by VX-809 and suggest multiple agents for full correction.

    • Farinha C
    • King-Underwood J
    • Sousa M
    • et al.
    N/AReaders
    N/ACitations
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Professional experience

Post-Doc Researcher

NOVA Medical School/CEDOC-Nova University of Lisbon

March 2013 - Present

Post-Doc

University of Lisboa/BioFIG-Faculty of Sciences

July 2012 - October 2012(3 months)

PhD Student

National Institute of Health/Genetics Dpt & University of Lisboa/BioFIG-Faculty of Sciences

January 2008 - July 2012(5 years)

PhD Student

State University of Campinas / Faculty of Medical Sciences

February 2010 - October 2010(8 months)

Junior Student

National Institute of Health/Genetics Dpt

September 2005 - December 2007(2 years)

Junior student

University of Regensburg / Institute of Physiology

January 2006 - July 2006(6 months)

Education history

PhD

Faculty of Sciences, Lisbon University

January 2008 - July 2012(5 years)

Licenciate

Instituto Politécnico de Lisboa - Polytechnic Institute of Lisbon

September 2004 - October 2005(a year)

Bachelor

Instituto Politécnico de Lisboa - Polytechnic Institute of Lisbon

September 2001 - July 2004(3 years)