The main focus of my research is invent of the highly automated and integrated instrumentation for the isolation of drugs and metabolites from complex matrices and the subsequent separation, identification and determination of these analytes. • Analysis of small and large molecules in biological fluids. • Screening and detection of biomarkers in CNS and Oncology diseases. • Quantification of drugs and metabolites in blood, plasma, urine and saliva. • Development of sample-preparation methods for bioanalysis including liquid-liquid extraktion (LLE), solid phase extraction (SPE), protein precipitation, solid-phase microextraction (SPME) and microextraction by packed sorbent (MEPS) for plasma and urine samples. • Development of new methods and validation of the methodology for determination of drugs and metabolites in biological fluids using GC-MS and LC-MS/MS. • Improvement of the separation and detection limits for gas chromatography –mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS-MS) by using interactive gases as carrier gas (GC) or as nebulizer gas in electrospray ionization with MS. I have published over 300 publications (peer-reviewed papers, proceedings, book and encyclopedia chapters, AstraZeneca internal research reports, patent, etc). I have supervised more than 28 M.Sc and Ph.D degrees. I have presented my research results in different international conferences as oral and poster presentations. Also, I have been as session chairman in different international conferences.
Application of graphitic sorbent for online microextraction of drugs in human plasma samples