A 13-lipoxygenase is Expressed Early in the Hypersensitive Reaction of Cotton Plants to Xanthomonas campestris pv. malvacearum

  • Sanier C
  • Sayegh-Alhamdia M
  • Jalloul A
 et al. 
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Abstract

Lipoxygenases (LOXs) are enzymes responsible for lipid peroxidation processes during plant defence responses to pathogen infection. Jasmonates are lipid-derived signals that mediate plant stress responses with chloroplastic LOXs implicated in the biosynthesis of oxylipins like jasmonic acid (JA). Hypersensitive reaction (HR) cell death of cotton to the incompatible race 18 of Xanthomonas campestris pathovar malvacearum (Xcm) is associated with 9S-lipoxygenase activity and expression of a 9-LOX GhLOX1. Here, we report the cloning of cotton (Gossypium hirsutum L.) LOX gene GhLOX2. Sequence analysis showed that GhLOX2 is a putative 13-LOX with a chloroplast-transit peptide in the amino acid terminus. GhLOX2 was found to be significantly expressed in the first hour of Xcm-induced HR. Investigation into LOX signalization on cotyledons incubated with methyl-jasmonate (MeJA) or infiltrated with salicylic acid (SA) or ethylene (ET) revealed that the first two treatments induced GhLOX2 gene expression. Our results show that GhLOX2 gene expression occurred at the stage of the HR prior biochemical events previously highlighted. The role that GhLOX2 may have in the defence strategy of cotton to Xcm is discussed regarding the HR.

Author-supplied keywords

  • Bacterial blight
  • Ethylene
  • Gossypium hirsutum
  • Hypersensitive reaction
  • Jasmonic acid
  • Lipoxygenase
  • Salicylic acid
  • Xanthomonas

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Authors

  • Christine Sanier

  • Majd Sayegh-Alhamdia

  • Aïda Jalloul

  • Alain Clerivet

  • Michel Nicole

  • Philippe Marmey

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