5'CATGCAT-3' Elements Modulate the Expression of Glycinin Genes.

  • Lelievre J
  • Oliveira L
  • Nielsen N
  • 14

    Readers

    Mendeley users who have this article in their library.
  • 58

    Citations

    Citations of this article.

Abstract

Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a beta-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT-3' element 101 base pairs upstream from the transcription start site in the construction caused about a 10-fold reduction in the amount of beta-glucuronidase activity compared with when the element was present in the gene. Elimination of 1.9 kilobase pairs from the 5'-end of the promoter caused a two-to threefold reduction in activity. The results show that the 5'-CATGCAT-3' element plays a role in regulating the amount of expression from the gene, but that there are also other factors farther upstream from the gene that affect the level of expression.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • J M Lelievre

  • L O Oliveira

  • N C Nielsen

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free