Accurate proteome-wide protein quantification from high-resolution N-15 mass spectra

  • Khan Z
  • Amini S
  • Bloom J
 et al. 
  • 3


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of N-15-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing N-15 spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Zia Khan

  • Sasan Amini

  • Joshua S Bloom

  • Cristian Ruse

  • Amy A Caudy

  • Leonid Kruglyak

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free