Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes

  • Li G
  • Liang Q
  • Gong P
 et al. 
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Abstract

We report a new class of deubiquitinating enzyme (DUB) probes that resemble the native diubiquitin with a same linkage size and contain a Michael addition acceptor for trapping the DUB active-site cysteine. Both K63- and K48-linked diubiquitin probes were generated using a facile chemical ligation method. The diUb probes were demonstrated to label DUBs from different families and revealed intrinsic linkage specificities of DUBs.

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Authors

  • Guorui Li

  • Qin Liang

  • Ping Gong

  • Adam H. Tencer

  • Zhihao Zhuang

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