Alginate microspheres of Bacillus subtilis

Abstract

Bacillus subtilis microspheres were prepared by using sodium alginate. Some typical properties of microencapsulated systems such as content of microorganisms, particle size and germination time were studied. Microspheres are prepared by the coacervation phase separation method, mainly based on an intermediate stage of primary emulsion. The preparation conditions were very soft to avoid changes in general biological products of calcium alginate microspheres containing cells, but giving them protection with a hydrogel matrix, without preventing the communication with the surrounding medium. The encapsulation of microbial spores and viable cells of a model microorganism (Bacillus subtilis) can be achieved by using sodium alginate in a satisfactory manner. Spore microspheres showed higher viability comparated to vegetative microspheres.