The selective inhibition of neuronal Shaw2 K+ channels by 1-alkanols is conferred by the internal S4-S5 loop, a region that also contributes to the gating of voltage-gated K+ channels. Here, we applied alanine scanning mutagenesis to examine the contribution of the S5 and S6 segments to the allosteric modulation of Shaw2 K+ channels by 1-alkanols. The internal section of S6 is the main activation gate of K + channels. While several mutations in S5 and S6 modulated the inhibition of the channels by 1-butanol and others had no effect, a single mutation at a key site in S6 (P410A) converted this inhibition into a dramatic dose-dependent potentiation (∼2-fold at 15 mM and ∼6-fold at 50 mM). P410 is the second proline in the highly conserved PVP motif that may cause a significant α-helix kink. The P410A currents in the presence of 1-butanol also exhibited novel kinetics (faster activation and slow inactivation). Internal application of 15 mM 1-butanol to inside-out patches expressing P410A did not significantly affect the mean unitary currents (∼2 pA at 0 mV) or the mean open time (5-6 ms) but clearly increased the opening frequency and open probability (∼2- to 4-fold). All effects displayed a fast onset and were fully reversible upon washout. The results suggest that the allosteric modulation of the Shaw2 K+ channel by 1-alkanols depends on a critical link between the PVP motif and activation gating. This study establishes the Shaw2 K+ channel as a robust model to investigate the mechanisms of alcohol intoxication and general anesthesia.
CITATION STYLE
Harris, T., Graber, A. R., & Covarrubias, M. (2003). Allosteric modulation of a neuronal K+ channel by 1-alkanols is linked to a key residue in the activation gate. American Journal of Physiology - Cell Physiology, 285(4 54-4). https://doi.org/10.1152/ajpcell.00113.2003
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