analysis of key molecules of the innate immune system in mammary epithelial cells isolated from marker - assisted and conventionally selected cattle

  • Griesbeck - Zilch B
  • Kühn C
  • Bruckmaier H
 et al. 
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Abstract

See, stats, and : https : / / www . researchgate . net / publication / 26761066 Analysis system from - assisted selected Article Impact : 2 . 57 : 10 . 3168 / jds . 2008 - 1954 : PubMed CITATIONS 32 READS 30 9 , including : Christa Leibniz 134 , 175 SEE Michael Technische 204 , 242 SEE All - text , letting . Available : Michael Retrieved : 14 aBStraCt Mastitis is the most prevalent infectious disease in dairy herds . Breeding programs considering mastitis susceptibility were adopted as approaches to improve udder health status . In recent decades , conventional selection criteria based on phenotypic characteristics such as somatic cell score in milk have been widely used to select animals . Recently , approaches to incorporate molecular information have become feasible because of the detection of quantitative trait loci (QTL) af - fecting mastitis resistance . The aims of the study were to explore molecular mechanisms underlying mastitis resistance and the genetic mechanisms underlying a QTL on Bos taurus chromosome 18 found to influ - ence udder health . Primary cell cultures of mammary epithelial cells from heifers that were selected for high or low susceptibility to mastitis were established . Se - lection based on estimated pedigree breeding value or on the basis of marker - assisted selection using QTL information was implemented . The mRNA expression of 10 key molecules of the innate immune system was measured using quantitative real - time PCR after 1 , 6 , and 24 h of challenge with heat - inactivated mastitis pathogens (Escherichia coli and Staphylococcus aureus) and expression levels in the high and low susceptibility groups were compared according to selection criteria . In the marker - assisted selection groups , mRNA expres - sion in cells isolated from less - susceptible animals was significantly elevated for toll - like receptor 2 , tumor ne - crosis factor - α , IL - 1β , IL - 6 , IL - 8 , RANTES (regulated upon activation , normal t - cell expressed and secreted) , complement factor C3 , and lactoferrin . In the estimated pedigree breeding value groups , mRNA expression was significantly elevated only for V -rel

Author-supplied keywords

  • dairy cattle
  • mammary epithelial cell
  • mastitis
  • quantitative trait locus

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Authors

  • B Griesbeck - Zilch

  • Ch Kühn

  • H Bruckmaier

  • W pfaffl

  • Hammerle - Fickinger

  • H H D meyer

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