Analysis of ??-lactalbumin, ??-lactoglobulin A and B in whey protein powder, colostrum, raw milk, and infant formula by CE and LC

  • Ding X
  • Yang Y
  • Zhao S
 et al. 
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Abstract

Two analytical methods, capillary electrophoresis (CE) with UV detection and high-performance liquid chromatography (HPLC) with photodiode array detection, were developed for the determination of +¦-lactalbumin (+¦-Lac), +¦-lactoglobulin A (+¦-Lg A), and +¦-lactoglobulin B (+¦-Lg B) in whey protein powder, colostrum, raw milk, and infant formula. The CE analysis was performed in a bare fused silica capillary (57 cm+ù50 ++m, effective length 50 cm) with a separation buffer consisting of 0.5 mol L-1 boric acid, 0.025 mol L-1 hydroxypropyl-+¦-cyclodextrin, and 0.8 g L-1 poly(ethylene oxide) 4,000,000 at pH 9.10. The HPLC analysis was performed on a CAPCELL PAK C8 SG 300 (200+ù4.6 mm, 5-++m particles) column with a gradient mobile phase mixture consisting of acetonitrile and water containing trifluoroacetic acid. The CE method is suitable for the simultaneous determination of +¦-Lac, +¦-Lg A, and +¦-Lg B in whey powder, colostrum, and raw bovine milk, whereas the HPLC method can only be used for quantitative assays of +¦-Lg A and +¦-Lg B due to the co-elution of lactoferrin with +¦-Lac. Recoveries for both methods over the concentration range were between 90.7% and 116.8%. Whey protein powder had the highest content of the above three proteins. The contents of +¦-Lac in infant formula increased with the raise of age stage. Infant formula with the same age stage but different brands and countries had significant differences in the content of +¦-Lac. -¬ INRA and Springer Science+Business Media B.V. 2011

Author-supplied keywords

  • ??-Lactalbumin
  • ??-Lactoglobulin
  • Capillary electrophoresis
  • High-performance liquid chromatography
  • Infant formula

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Authors

  • Xiaojing Ding

  • Yuanyuan Yang

  • Shan Zhao

  • Yun Li

  • Zhi Wang

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