Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with CoulArray detection

  • Beňová B
  • Adam M
  • Onderková K
 et al. 
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The roots of three varieties of Polygonum cuspidatum were analyzed for resveratrol and its analogs. The powder of the dried roots was extracted with aqueous ethanol (60% v/v) and the extracts obtained were analyzed using RP HPLC with coulometric detection. A simple HPLC method with a multichannel CoulArray detector was developed for the determination of four stilbenes: resveratrol, its glucoside piceid, piceatannol, and its glucoside astringin. Analyses were carried out on a LiChrospher C18 (125 x 4.6 mm id, particle size 5 microm) column with a mobile phase of ammonium acetate (pH 3) and ACN in gradient mode. Four compounds were monitored by a CoulArray electrochemical detector. Potentials of eight electrochemical cells in series were set in the range of 200-900 mV. Optimization of the mobile phase pH was performed. Calibration curves showed good linearity with correlation coefficients (r(2))--more than 0.9975.

Author-supplied keywords

  • Electrochemical detection
  • HPLC
  • Polygonum cuspidatum
  • Stilbenes

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  • Blanka Beňová

  • Martin Adam

  • Kateřina Onderková

  • Josef Královský

  • Milan Krajíček

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