Antipsychotic drugs inhibit nucleotide hydrolysis in zebrafish (Danio rerio) brain membranes

  • Seibt K
  • Oliveira R
  • Rico E
 et al. 
  • 1


    Mendeley users who have this article in their library.
  • 17


    Citations of this article.


Haloperidol (HAL), olanzapine (OLZ), and sulpiride (SULP) are antipsychotic drugs widely used in the pharmacotherapy of psychopathological symptoms observed in schizophrenia or mood-related psychotic symptoms in affective disorders. Here, we tested the in vitro effects of different concentrations of a typical (HAL) and two atypical (OLZ and SULP) antipsychotic drugs on ectonucleotidase activities from zebrafish brain membranes. HAL inhibited ATP (28.9%) and ADP (26.5%) hydrolysis only at 250 μM. OLZ decreased ATPase activity at all concentrations tested (23.8-60.7%). SULP did not promote significant changes on ATP hydrolysis but inhibited ADP hydrolysis at 250 μM (25.6%). All drugs tested, HAL, OLZ, and SULP, did not promote any significant changes on 5′-nucleotidase activity in the brain membranes of zebrafish. These findings demonstrated that antipsychotic drugs could inhibit NTPDase activities whereas did not change 5′-nucleotidase. Such modulation can alter the adenosine levels, since the ectonucleotidase pathway is an important source of extracellular adenosine. Thus, it is possible to suggest that changes promoted by antipsychotic drugs in the bilayer membrane could alter the NTPDase activities, modulating extracellular ATP and adenosine levels. © 2008 Elsevier Ltd. All rights reserved.

Author-supplied keywords

  • Antipsychotic
  • Ectonucleotidase
  • Haloperidol
  • Olanzapine
  • Schizophrenia
  • Sulpiride
  • Zebrafish

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Kelly Juliana Seibt

  • Renata da Luz Oliveira

  • Eduardo Pacheco Rico

  • Renato Dutra Dias

  • Mauricio Reis Bogo

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free