The application of fixed hydrophobic patterns for confinement of aqueous solutions in proteomic microarrays

  • Li Y
  • McKenna E
  • Parkes W
 et al. 
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A protein microarray hybridisation system has been implemented by employing patterned hydrophobic thin films on hydrophilic substrates as a means of confinement for aqueous samples. This approach has the ability to handle, and keep separate, small sample volumes of just a few microlitres. In addition, the system is more straightforward to use than the existing multi-well gasket solution. The paper describes the fabrication method and the system is demonstrated for a model protein microarray assay. © 2011 American Institute of Physics.

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