Assessment of the modulating effects of fructo-oligosaccharides on fecal microbiota using human flora-associated piglets

  • Shen J
  • Zhang B
  • Wei H
 et al. 
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Abstract

We first used human flora-associated (HFA) piglets, a significantly
improved model for research on human gut microbiota, to study the
effects of short-chain fructo-oligosaccharides (scFOS) on the gut
bacterial populations. Ten neonatal HFA piglets were assigned to
receive basal diets alone or supplemented with scFOS (0.5?g/kg body
weight/day) from 1 to 37?days after birth (DAB). The impact of scFOS
on the fecal bacterial populations of the piglets before (12 DAB),
during (17 DAB), and after (25 and 37 DAB) weaning were monitored
by PCR-denaturing gradient gel electrophoresis and real-time quantitative
PCR. The Bifidobacterium genus was stimulated consistently, except
during weaning, confirming the bifidogenic property of scFOS. At
12 DAB, the Clostridium leptum subgroup was decreased and two unknown
Bacteroides-related species were increased; at 25 DAB, the C. leptum
subgroup and Subdoligranulum variabile-like species were elevated,
whereas one unknown Faecalibacterium-related species was suppressed;
and at 37 DAB, the Bacteroides genus was decreased. The results showed
that effects of scFOS on non-bifidobacteria varied at different developmental
stages of the animals, warranting further investigation into the
host-development-related effects of prebiotics on the gut microbiota
and the host physiology using the HFA piglets as a model for humans.

Author-supplied keywords

  • Denaturing gradient gel electrophoresis (DGGE)
  • Fructo-oligosaccharides
  • Human flora-associated piglet model
  • Intestinal microbiota
  • Prebiotics
  • Real-time PCR

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Authors

  • Jian Shen

  • Baorang Zhang

  • Hua Wei

  • Chuanyan Che

  • Dezhong Ding

  • Xiuguo Hua

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