Atomic force microscopy comes of age

  • Francis L
  • Lewis P
  • Wright C
 et al. 
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Abstract

AFM (atomic force microscopy) analysis, both of fixed cells, and live cells in physiological environments, is set to offer a step change in the research of cellular function. With the ability to map cell topography and morphology, provide structural details of surface proteins and their expression patterns and to detect pico-Newton force interac-tions, AFM represents an exciting addition to the arsenal of the cell biologist. With the explosion of new applications, and the advent of combined instrumentation such as AFM–confocal systems, the biological application of AFM has come of age. The use of AFM in the area of biomedical research has been proposed for some time, and is one where a significant impact could be made. Fixed cell analysis provides qualitative and quantitative subcellular and surface data capable of revealing new biomarkers in medical pathologies. Image height and contrast, surface roughness, fractal, volume and force analysis provide a platform for the multiparameter analysis of cell and protein functions. Here, we review the current status of AFM in the field and discuss the important contribution AFM is poised to make in the understanding of biological systems. Introduction AFM (atomic force microscopy) is an exciting ana-lytical tool that was originally developed for the characterization of nanometre-scaled semiconductor devices, but is increasingly used in biological and biophysical research due to its unique analytic cap-abilities (reviewed in Parot et al., 2007). Since its inception there has been a pioneering decade of in-tense instrument development followed by improve-ments in sample preparation (El Kirat et al., 2005), interpretation (Horber and Miles, 2003) and data ana-lysis (Horber, 2002). The ability of AFM to generate image contrast, utilizing its unique contact probe methodology, is becoming an established method in cell biology (for a detailed technology review, see Gerber and Lang, 2006; detailed information has been gathered from a variety of cell types in the fol-lowing: Parpura et al., 1993; Ohnesorge et al., 1997; Butt et al., 2007). Its potential for high sensitiv-ity, high-throughput operation in fluid, and for force 1 To whom correspondence should be addressed (email fran1@hotmail.com).

Author-supplied keywords

  • AFM
  • CLSM
  • HCA
  • SCFS
  • SEM
  • SMFS
  • atomic force microscopy
  • atomic force microscopy (AFM)
  • confocal laser scanning microscopy
  • force interaction
  • hierarchical cluster analysis
  • imaging
  • scanning electron microscopy
  • single cell force spectroscopy
  • single molecule force spectroscopy
  • surface topography
  • systems biology Abbreviations used

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Authors

  • Lewis W Francis

  • Paul D Lewis

  • Chris J Wright

  • R Steve Conlan

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