Automated enzymatic standardized lipid analyses for plasma and lipoprotein fractions

  • McNamara J
  • Schaefer E
  • 14

    Readers

    Mendeley users who have this article in their library.
  • 385

    Citations

    Citations of this article.

Abstract

Excellent normal ranges for plasma lipid and lipoprotein cholesterol levels have been developed by the Lipid Research Clinics program, standardized by the Centers for Disease Control (CDC). However these values were generated by methods not currently in use in most clinical chemistry laboratories. Automated enzymatic methods for cholesterol, triglycérides and free glycerol determinations, as well as a dextran sulfate-Mg2+procedure for separation of high density lipoproteins (HDL) with standardization are described. Similar methods for the measurement of unesterified cholesterol and phospholipids are also given. Serum pools for total cholesterol with values ranging from 1220-3490 mg/l produced coefficients of variation (CV) ≤2.85%; reference values for low total cholesterol samples in a range of 280-727 mg/l gave CV of 4.35% or less; HDL cholesterol reference values of 265-640 mg/l yielded CV ≤3.70%; and values for triglycerides between 0.74 and 3.05 mmol/l gave CV of 4.22% or less through three to eight testing cycles (9-24 mth). These data indicate that long term CDC standardization of total cholesterol, triglycerides, and HDL cholesterol can be obtained with automated enzymatic methods utilizing commercially available reagents. © 1987.

Author-supplied keywords

  • Cholesterol
  • Cholesterol ester
  • Glyceride
  • HDL
  • LDL
  • Lipid
  • Lipoprotein
  • Phospholipid
  • Triglyceride
  • VLDL

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Judith R. McNamara

  • Ernst J. Schaefer

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free