Autoradiography using storage phosphor technology

  • Johnston R
  • Pickett S
  • Barker D
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Abstract

We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32P, 14C and 35S. Imaging plates can detect a 32P-labeled sample at an exposure level of 1 disintegration/mm2, and 14C and 35S at 25 disintegrations/mm2. Compared to X-ray film, imaging plates offer increased sensitivity of 15- to 250-fold for 32P and 20- to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.

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Authors

  • Richard F. Johnston

  • Siobhan C. Pickett

  • David L. Barker

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