B cell intrinsic MyD88 signals drive IFN-gamma production from T cells and control switching to IgG2c.

  • Barr T
  • Brown S
  • Mastroeni P
 et al. 
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The question of whether Ab responses to T-dependent Ags require B cell intrinsic signaling via the main TLR adaptor (MyD88) has become embroiled in confusion. In part this may be related to the methods used to analyze B cell intrinsic signaling. We have used a mixed bone marrow chimera model to generate mice in which the B cell compartment is completely deficient in MyD88 expression, while the other hematopoietic lineages are largely normal. These mice were immunized with T-dependent Ags or infected with Salmonella. We found that the Ag-specific IgG2c primary response was absolutely dependent on MyD88 signaling to B cells, while other Ig classes were not (IgG1 and IgG3) or much less so (IgG2b, IgA). The MyD88(B-/-) chimeric mice exhibited an impairment of development of IFN-gamma effector T cells, a likely contributory factor in the lack of IgG2c. We also found that B cell intrinsic MyD88 signals are required for the production of natural Abs. The data emphasize the nonredundant role of B cells as programmers of T cell differentiation in vivo.

Author-supplied keywords

  • Animals
  • Antibody Formation
  • Antibody Formation: immunology
  • B-Lymphocytes
  • B-Lymphocytes: immunology
  • Cell Differentiation
  • Cell Differentiation: immunology
  • Immunoglobulin Class Switching
  • Immunoglobulin G
  • Interferon-gamma
  • Interferon-gamma: biosynthesis
  • Mice
  • Myeloid Differentiation Factor 88
  • Myeloid Differentiation Factor 88: metabolism
  • Signal Transduction
  • T-Lymphocytes
  • T-Lymphocytes: cytology
  • T-Lymphocytes: metabolism

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  • Tom a Barr

  • Sheila Brown

  • Pietro Mastroeni

  • David Gray

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