Biological monitoring method for urinary neonicotinoid insecticides using LC-MS/MS and its application to Japanese adults

  • Ueyama J
  • Nomura H
  • Kondo T
 et al. 
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Abstract: Biological Monitoring Method for Urinary Neonicotinoid Insecticides Using LC-MS/MS and Its Application to Japanese Adults: Jun UEYAMA, et al. Department of Pathophysiological Laboratory Sciences, Field of Radiological and Medical Labora- tory Sciences, Nagoya University Graduate School of Medicine—Objectives: Agricultural use of neonic- otinoid (NEO) insecticides has been increasing in recent years, but their biological monitoring methods have been scarcely reported. In this study, we developed and validated a rapid and sensitive method for quantifying urinary NEO concentrations using high-performance liquid chromatography-tandem mass spectrometry (LC- MS/MS). Methods: After phosphate-induced acidifica- tion of a urine sample, urinary NEOs were trapped by a solid-phase extraction column and eluted with meth- anol for acetamiprid, imidacloprid, thiacloprid, thiameth- oxam, clothianidin and dinotefuran and with an aceto- nitrile and methanol solution (1:1, v/v) containing 5% NH3 for nitenpyram. A separation analysis was performed by LC-MS/MS within 10 minutes for the sample. This method was applied to first morning urine obtained from 52 Japanese (40.9 ± 10.5 years old, mean ± standard deviation) without occupational NEO exposure. Results: The linear dynamic ranges and their limit of quantification (LOQ, signal to noise ratio=10) levels were 0.3−20 or 50 μ g/l (r =0.998−0.999) and 0.05−0.36 μ g/l, respectively. The absolute recovery was 64−95%, and the intra- and inter-day precisions were less than 16.4% (relative standard deviation, %RSD). This method was successfully applied for analysis of NEOs in human urine samples obtained from 52 adults. The frequencies of individuals who showed more than LOD levels was above 90% for imidacloprid, thiamethoxam, clothianidin and dinotefuran, more than 50% for acetamiprid and thiacloprid and 29% for nitenpyram. Conclusions: These results indicated that our new method could be applied to biological monitoring of NEO exposure even at environmental exposure levels in Japanese adults without occupational spraying histories.

Author-supplied keywords

  • Biological monitoring
  • LC-MS/MS
  • Neonicotinoid
  • Urine

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  • Jun Ueyama

  • Hiroshi Nomura

  • Takaaki Kondo

  • Isao Saito

  • Aya Osaka

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