A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.
CITATION STYLE
Arenz, B. E., Schlatter, D. C., Bradeen, J. M., & Kinkel, L. L. (2015). Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities. Journal of Microbiological Methods, 117, 1–3. https://doi.org/10.1016/j.mimet.2015.07.003
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