Calcitonin gene-related peptide elevates cyclic AMP levels in chick skeletal muscle: possible neurotrophic role for a coexisting neuronal messenger.

  • Laufer R
  • Changeux J
  • 7


    Mendeley users who have this article in their library.
  • 152


    Citations of this article.


Recent immunocytochemical studies have shown that calcitonin gene-related peptide (CGRP) coexists with the neurotransmitter acetylcholine in spinal motoneurons of the chick. Moreover, CGRP causes an increase in the number of acetylcholine receptors on the surface of cultured chick myotubes. CGRP might thus serve as one of the signals by which motoneurons regulate endplate development. In a search for the second messengers involved, we now demonstrate that CGRP stimulates accumulation of cyclic AMP (cAMP) in cultured chick myotubes. This effect is, at least in part, mediated by an increase in cAMP synthesis, as the peptide also activates adenylate cyclase in chick muscle membranes. Nanomolar concentrations of CGRP elicit significant increases in both cellular cAMP levels and acetylcholine receptor numbers. The present findings suggest that cAMP is one of the second messengers which mediate the increase in acetylcholine receptor number elicited by CGRP. Furthermore, CGRP might be implicated in other trophic actions mediated by cAMP in skeletal muscle cells.

Author-supplied keywords

  • Adenylyl Cyclases
  • Adenylyl Cyclases: metabolism
  • Animals
  • Calcitonin Gene-Related Peptide
  • Cell Membrane
  • Cell Membrane: metabolism
  • Cells, Cultured
  • Chick Embryo
  • Cyclic AMP
  • Cyclic AMP: metabolism
  • Muscles
  • Muscles: drug effects
  • Muscles: innervation
  • Muscles: metabolism
  • Neurons
  • Neurons: physiology
  • Neuropeptides
  • Neuropeptides: pharmacology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

  • ISSN: 0261-4189
  • PUI: 17761462
  • SGR: 0023323998
  • SCOPUS: 2-s2.0-0023323998
  • PMID: 3036493


  • R Laufer

  • J P Changeux

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free