Four males and nine females Bufo spinulosus arequipensis were analyzed in order to describe the cariotype with the conventional Giemsa sequential colouring technique, followed by C Banding, and to obtain the C Banding ideogram. The specimens had a diploid number 2n = 22 and an haploid number n = 11 chromosomes. All chromosomic pairs have zones of centromeric constitutive heterochromatin.
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