CD spectroscopy of proteins adsorbed at flat hydrophilic quartz and hydrophobic teflon surfaces

  • Vermeer A
  • Norde W
  • 61

    Readers

    Mendeley users who have this article in their library.
  • 35

    Citations

    Citations of this article.

Abstract

Spectroscopic methods provide a powerful tool for studying the properties of proteins at interfaces. The protein accumulated in one adsorbed layer is frequently less than the minimum mass of protein required by a detection method. In such a case (as is the case in circular dichroism spectroscopy) the sorbent material is usually supplied as dispersion. However, light scattering by the dispersed particles often interferes with the measurement of the circular dichroism of the protein. Therefore, there is a strong need for an experimental setup that enables these measurements to be made using flat surfaces. An example of such a setup is the multiplate quartz cell presented here. The potential of this multiplate quartz cell is shown by some preliminary circular dichroism measurements of IgG adsorbed on different types of surfaces. (C) 2000 Academic Press.

Author-supplied keywords

  • CD measurements
  • Protein adsorption
  • Structural changes
  • Teflon

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free