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Journal article

Cells respond to mechanical stress by rapid disassembly of caveolae

Sinha B, Köster D, Ruez R, Gonnord P, Bastiani M, Abankwa D, Stan R, Butler-Browne G, Vedie B, Johannes L, Morone N, Parton R, Raposo G, Sens P, Lamaze C, Nassoy P ...see all

Cell, vol. 144, issue 3 (2011) pp. 402-413

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Abstract

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin- and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin- and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses. PaperFlick: © 2011 Elsevier Inc.

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