The cellulase of Trichoderma viride Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and β‐glucosidases

  • BELDMAN G
  • SEARLE‐VAN LEEUWEN M
  • ROMBOUTS F
 et al. 
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Abstract

1. Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C1) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions.

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Authors

  • Gerrit BELDMAN

  • Marjo F. SEARLE‐VAN LEEUWEN

  • Frank M. ROMBOUTS

  • Fons G.J. VORAGEN

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