Changes in membrane microviscosity associated with phagocytosis: effects of colchicine.

  • Berlin R
  • Fera J
  • 9

    Readers

    Mendeley users who have this article in their library.
  • 94

    Citations

    Citations of this article.

Abstract

The effects of phagocytosis on plasma membrane microviscosity were studied by fluorescence depolarization techniques. It was shown that lipophilic probes are accumulated in intracellular vesicles to a significant degree in fibroblasts and neutrophils. Microviscosity was thus determined from the behavior of probes in isolated membranes. Phagocytosis of oil emulsions or polystyrene beads by rabbit polymorphonuclear leukocytes induces a marked decrease in plasma membrane microviscosity that parallels the extent of phagocytosis. Liposomes made from extracts of membrane lipid show qualitatively the same changes, indicating that the alteration of microviscosity results at least in part from changes in lipid composition. The decrease in microviscosity is abolished when colchicine is present during phagocytosis. Addition of colchicine to membranes previously isolated from control or phagocytic cells has no effect on their microviscosity. The results suggest that phagocytosis is accompanied by a microtubule-dependent reorganization of membrane lipids.

Author-supplied keywords

  • Animals
  • Cell Membrane
  • Cell Membrane: physiology
  • Colchicine
  • Colchicine: pharmacology
  • Fibroblasts
  • Fluorescent Dyes
  • Liposomes
  • Microscopy, Fluorescence
  • Microtubules
  • Microtubules: physiology
  • Neutrophils
  • Phagocytosis
  • Phagocytosis: drug effects
  • Rabbits
  • Viscosity

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • R D Berlin

  • J P Fera

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free