Characterization of an Established Line of Canine Kidney Cells (MDCK)

Abstract

An established line of canine kidney cells (MDCK-USD) of male origin was shown to contain 3 metacentric chromosomes and possess a modal number of 81. It grew rapidly in Eagle's MEM, displayed contact inhibition and was susceptible to vaccinia, VSV and influenza (PR8) viruses. Two additional MDCK lines, the parent line and another derivative from other laboratories, were compared to the USD line and could be distinguished on the basis of modal number and morphology of the metacentric chromosomes observed in each line. © 1966, SAGE Publications. All rights reserved.