Characterization of hepatitis B virus reverse transcriptase sequences in Chinese treatment naive patients.

  • Han Y
  • Huang L
  • Liu C
 et al. 
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Abstract

BACKGROUND AND AIMS: The hepatitis B virus (HBV) reverse transcriptase
(RT) plays an important role in viral replication. The aim of the
present study was to characterize profiles of the RT region and to
construct a database for further studies. METHODS: Serum samples
were obtained from 328 treatment naive patients chronically infected
with HBV in five Chinese cities. Mutation status, genotypes and deep
sequence analysis were carried out by amplifying and sequencing the
RT region. RESULTS: The base usage in the RT region differed at the
mono- and dinucleotide level and thymidine dominated. The higher
the variability of the strain was, the more it replicated. No significant
clustering was found between our HBV RT sequences and those isolated
10 years ago (achieved from genebank). Nucleotide analogue resistance
related mutants exist. The M204V/I mutation was found in 1.8% of
the strains, 1.2% had L180M+ M204V/I, 0.6% had A181T/V, and only
one had all three mutations. Minor strain mutants were found in 9.3%
of the samples studied. The genotype B patients made up 36.6% (88.7%
B2) and were mostly found in southern China, 63.4% (92.2% C2) were
genotype C, and only one was genotype D. The average age of HBeAg
positive genotype B patients was 29.5 +/- 10.4 years, for genotype
C it was 36.1 +/- 10.9 (P < 0.001). CONCLUSION: Primarily antiviral
resistance related mutant strains do exist in treatment na�ve patients.
Without antiviral pressure, HBV strains evolved at a normal speed.
In depth sequence analysis implied that viral replication might be
correlated with its variability, which needs to be further investigated.

Author-supplied keywords

  • Adenine
  • Chronic
  • Genetic; Drug Resistance
  • Viral
  • analogs /&/ derivatives/therapeutic use; Adult; A
  • analogs /&/ derivatives/therapeutic use; Hepatiti
  • blood; Databases
  • blood; Hepatitis B virus
  • diagnosis/drug therapy/ethnology; Humans; Lamivud
  • drug effects/enzymology/genetics/immunology; Hepa
  • epidemiology; Computational Biology; DNA
  • genetics; Female; Genotype; Guanine
  • genetics; Treatment Failure; Virus Replication
  • genetics; Young Adult
  • therapeutic use; Asian Continental Ancestry Group
  • therapeutic use; Male; Middle Aged; Mutation; Phe
  • therapeutic use; Phylogeny; RNA-Directed DNA Poly

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Authors

  • Yue Han

  • Li Hua Huang

  • Chuan Miao Liu

  • Shu Yang

  • Juan Li

  • Zhi Mei Lin

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