Characterization and quantification of major constituents of Xue Fu Zhu Yu by UPLC-DAD-MS/MS

  • Zhang L
  • Zhu L
  • Wang Y
 et al. 
  • 11


    Mendeley users who have this article in their library.
  • 24


    Citations of this article.


Xue Fu Zhu Yu (XFZY), a classic recipe in traditional Chinese medicine (TCM), has been demonstrated to show protective effects on cardiovascular system. For quality control of XFZY products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector-tandem mass spectrometry (UPLC-DAD-MS) was undertaken. Twenty-eight compounds from XFZY were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds were tentatively characterized. According to qualitative results, a new method for quantitative analysis of XFZY has been established by ultra high performance liquid chromatography coupled with diode array detector (UPLC-DAD). Twelve representative compounds unequivocally identified were used as chemical markers in quantitative analysis, including 5-hydroxymethyl-2-furaldehyde (5-HMF), hydroxysafflor yellow A (HSYA), amygdalin, albiflorin, paeoniflorin, liquiritin, ferulic acid (FA), naringin, hesperidin, neohesperidin (NH), isoliquiritigenin (IL) and glycyrrhizic acid (GA), which were derived from nine of eleven herbs of XFZY except Platycodon grandiflorum (Jacq.) A. DC. (Jiegeng) and Bupleurum chinense DC. (Chaihu). This UPLC method was validated in terms of linearity, LOD and LOQ, precision, repeatability, stability, and recovery tests. Quality control of XFZY products in total fourteen samples by four dosage forms was examined by this method, and results confirmed its feasibility and reliability in practice. © 2011 Elsevier B.V.

Author-supplied keywords

  • Qualitative analysis
  • Quantitative analysis
  • Xue Fu Zhu Yu

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Lei Zhang

  • Lin Zhu

  • Yuefei Wang

  • Zhenzuo Jiang

  • Xin Chai

  • Yan Zhu

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free