Characterization of two protein kinases from Schizosaccharomyces pombe involved in the regulation of DNA repair

  • Dhillon N
  • Hoekstra M
  • 6


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


We have identified two novel genes designated hhp1+ and hhp2+ in the fission yeast Schizosaccharomyces pombe. The hhp1+ and hhp2+ genes encode two closely related protein kinases that share significant sequence identities with Hrr25p from Saccharomyces cerevisiae. Characterization of strains harboring single and double mutations in the hhp+ genes reveals DNA repair defects in these cells. Schizosaccharomyces pombe strains lacking either or both Hhp activities reveal differences in their ability to withstand DNA lesions caused by either methyl methanesulfonate (MMS) or gamma-rays which correlate with their ability to repair DNA strand breaks caused by these agents. We suggest that Hhp1 and Hhp2 are involved in the regulation of distinct and overlapping DNA repair pathways in S. pombe.

Author-supplied keywords

  • *Casein Kinase I
  • *DNA Repair
  • *Saccharomyces cerevisiae Proteins
  • *Schizosaccharomyces pombe Proteins
  • Amino Acid Sequence
  • Base Sequence
  • Casein Kinases
  • DNA, Fungal/radiation effects
  • Escherichia coli/genetics
  • Fungal Proteins/genetics
  • Gamma Rays
  • Genes, Fungal/*genetics
  • Genetic Complementation Test
  • Isoenzymes/genetics
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Protein Kinases/*genetics/metabolism
  • Recombinant Proteins/metabolism
  • Saccharomyces cerevisiae/genetics
  • Schizosaccharomyces/enzymology/*genetics
  • Sequence Homology, Amino Acid

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • N Dhillon

  • M F Hoekstra

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free