Characterization of two protein kinases from Schizosaccharomyces pombe involved in the regulation of DNA repair.

  • Dhillon N
  • Hoekstra M
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Abstract

We have identified two novel genes designated hhp1+ and hhp2+ in the fission yeast Schizosaccharomyces pombe. The hhp1+ and hhp2+ genes encode two closely related protein kinases that share significant sequence identities with Hrr25p from Saccharomyces cerevisiae. Characterization of strains harboring single and double mutations in the hhp+ genes reveals DNA repair defects in these cells. Schizosaccharomyces pombe strains lacking either or both Hhp activities reveal differences in their ability to withstand DNA lesions caused by either methyl methanesulfonate (MMS) or gamma-rays which correlate with their ability to repair DNA strand breaks caused by these agents. We suggest that Hhp1 and Hhp2 are involved in the regulation of distinct and overlapping DNA repair pathways in S. pombe.

Author-supplied keywords

  • Amino Acid
  • Amino Acid Sequence
  • Base Sequence
  • Casein Kinase I
  • Casein Kinases
  • DNA
  • DNA Repair
  • Escherichia coli
  • Escherichia coli: genetics
  • Fungal
  • Fungal Proteins
  • Fungal Proteins: genetics
  • Fungal: genetics
  • Fungal: radiation effects
  • Gamma Rays
  • Genes
  • Genetic Complementation Test
  • Insertional
  • Isoenzymes
  • Isoenzymes: genetics
  • Molecular Sequence Data
  • Mutagenesis
  • Protein Kinases
  • Protein Kinases: genetics
  • Protein Kinases: metabolism
  • Recombinant Proteins
  • Recombinant Proteins: metabolism
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae: genetics
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Schizosaccharomyces: enzymology
  • Schizosaccharomyces: genetics
  • Sequence Homology

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Authors

  • Namrita Dhillon

  • M F Hoekstra

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