In this study, we validated a thromboelastography (TEG) method to evaluate the hemostatic effects of 3 peptides. The first peptide is an ideal amphipathic peptide composed of 22 leucine and lysine in a ratio of 2:1. At a very low concentration, the peptide had a procoagulant effect shown by decreases in reaction time (R) and coagulation time (K) but was impaired by a decrease in maximum amplitude (MA). At higher concentrations, the peptide had an anticoagulant effect. The α angle was minimally affected by the peptide. The second peptide is melittin derived from bee venom. Melittin showed procoagulant effects reflected by a decrease in clotting time but led to lower MA. The third peptide derived from fibrinogen γ chain promoted hemostasis only at an optimal concentration and became anticoagulant at a higher concentration. The hemostatic mechanisms of each peptide were discussed. Our study would facilitate further development of peptides for either hemorrhage control or thrombosis treatment. © 2012 The Author(s).
CITATION STYLE
Peng, H. T., Blostein, M. D., & Shek, P. N. (2012). Characterization of in vitro hemostatic peptide effects by thromboelastography. Clinical and Applied Thrombosis/Hemostasis, 18(1), 27–34. https://doi.org/10.1177/1076029611412371
Mendeley helps you to discover research relevant for your work.